Glucocorticoids regulate renal glucocorticoid receptors and Na
Nike mercurial superfly red nike mercurial vapor x fg k ATPase activity
Demetrius Ellis1, Tran Dang Sothi1 and simply Ellis D Avner1
1Nephrology research you, Childrens Hospital of Pittsburgh, Department of pediatric medicine, University of Pittsburgh School of medication, Pittsburgh, Pa, American
Distance learning: Demetrius Ellis maryland, Manager of Nephrology, Baby’s Hospital of Pittsburgh, One little one’s Place, 3705 Fifth Avenue at DeSoto saint, Pittsburgh, California 152133417, The united states.
Obtain 16 October 1986; Adjusted 12 March 1987.
Top of pageAbstractK ATPase activity. Primary cultures of mouse renal tubular epithelial cells were used to study the effect of hydrocortisone on the dangerous glucocorticoid receptors(Gary) And on salt adenosine triphosphatase(NaK ATPase) Processes. A GR nike mercurial vapor x fg assay was made and performed directly on cell monolayers maintained in serum medium to which hydrocortisone at 5 nM, 50 nM, And 5 104 M was extra. Unlike control cells grown in medium without hydrocortisone, GR levels per cell decreased by 50% after 48 hours of growth in medium which contain 5 nM hydrocortisone. After 96 hours of cell treatment with supraphysiologic hydrocortisone concentrations of mit(50 nM or it may be 5 104 M), GR levels slashed to 28% of control values. In all hydrocortisone treatment groups there was an inverse relation between GR concentrations of mit and NaK ATPase activity. Binding of cell GR by adding the antiglucocorticoid RU 38486 in hydrocortisone medium eliminated the glucocorticoid stimulation of NaK ATPase activity. These results demonstrate a time and dose effect of glucocorticoids on GR binding activity and a direct relation between this receptor communication and interplay and NaK ATPase activity in intact renal tubular epithelial cells.
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